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All of the references cited above, as well as all references cited herein, are incorporated herein by reference in their entireties. The new chat rooms have emoticons, and private chat areas as well. The oligomers were synthesized using methods well known in the art.
Calcium oscillation reduction in the neuronal cells was measured for the oligomers of the invention and compared with that of the control cells (i.e., the calcium oscillations in the neuronal cells that are not treated with the oligomers). In certain embodiments, examples of the oligomers (i.e., antisense oligonucleotides) include, but are not limited to, the oligomers listed in FIGS.
The oligomers of the invention can therefore have at least one bicyclic nucleoside attached to the 3' or 5' termini by a neutral internucleoside linkage, such as one or more phosphotriester, methylphosphonate, MMI, amide-3, formacetal or thioformacetal.
Conjugation (to a conjugate moiety) can enhance the activity, cellular distribution or cellular uptake of the oligomer of the invention. The invention also provides for a conjugate comprising the oligomer according to the invention as herein described, and at least one non-nucleotide or non-polynucleotide moiety covalently attached to said oligomer.